Two groups of integrated vertical-flow constructed wetland (IVCW) microcosms were established for treating two types of representative wastewater: domestic and nitrified wastewater under two loading rates (LRs) over about two years. Their removal capacities of organic substance and nitrogen as well as the effects of loading rate (LR), outflow temperature and dissolved oxygen (DO) concentration were investigated and compared. Efficient chemical oxygen demand (COD) eliminations were achieved by the IVCWs, with the mass removal rates increasing linearly with the increasing LRs strongly, achieving average value of 56.07g m(-2) d(-1) at the highest loading rate. Nevertheless, the effluent COD concentrations also increased, with the average value exceeding Class I A discharge standard (< 50mg L-1) for municipal wastewater treatment plants in China at the highest loading rate. Greater total nitrogen (TN) mass removal rates but lower efficiencies were obtained at the high LR for both types of wastewater, and better removal was achieved for nitrified wastewater (NW) in comparison to domestic wastewater (DW), probably due to the prevailing anoxic conditions inside the IVCW beds restricted nitrification process of DW. The influences of LR, temperature and DO on COD removal were slight, but all remarkable on TN reduction. As compared to DO, temperature was more crucial for nitrogen removal, and the temperature dependence coefficient for TN removal of low LR of NW was significantly greater than others.