The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)

作者:	Le HAO, Ping XIE, Juan FU, Guangyu LI, Qian XIONG, Huiying LI
通讯作者:	Ping XIE
摘要:	The glutathione S-transferases play important roles in the detoxification of microcystin. Core-sequences of three classes of GST (mu, kappa and rho) were cloned from goldfish (Carassius auratus L.) i.p. injected with cyanobacterial crude extract at two doses (50 and 200g MC-LReq kg−1 BW). The relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GSTmu was inhibited in intestine at both doses and the transcription of GST kappa was inhibited from 12 to 48 h in kidney at both doses. The decreased transcription of GST rho was detected in all three organs at the high dose. It is suggested that transcription inhibition of GST rho might be significant in MCs toxicity at higher toxin concentration in omnivorous freshwater fish. Alteration in transcription of GSTs stimulated by MCs implicates an increased health risk to fish.
卷:	91
期:	1
页码:	99
发表时间:	2008-11-24
刊物名称:	AQUATIC TOXICOLOGY
全文链接:	http://dx.doi.org/10.1016/j.aquatox.2008.11.011